A germline-limited piggyBac transposase gene is required for precise excision in Tetrahymena genome rearrangement.

ABSTRACT: Developmentally programmed genome rearrangement accompanies differentiation of the silent germline micronucleus into the transcriptionally active somatic macronucleus in the ciliated protozoan Tetrahymena thermophila. Internal eliminated sequences (IES) are excised, followed by rejoining of MAC-destined sequences, while fragmentation occurs at conserved chromosome breakage sequences, generating macronuclear chromosomes. Some macronuclear chromosomes, referred to as non-maintained chromosomes (NMC), are lost soon after differentiation. Large NMC contain genes implicated in development-specific roles. One such gene encodes the domesticated piggyBac transposase TPB6, required for heterochromatin-dependent precise excision of IES residing within exons of functionally important genes. These con

SUBMITTER: Feng L 

PROVIDER: S-EPMC5766162 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature