Accumulation of histone variant H3.3 with age is associated with profound changes in the histone methylation landscape.

ABSTRACT: Deposition of replication-independent histone variant H3.3 into chromatin is essential for many biological processes, including development and reproduction. Unlike replication-dependent H3.1/2 isoforms, H3.3 is expressed throughout the cell cycle and becomes enriched in postmitotic cells with age. However, lifelong dynamics of H3 variant replacement and the impact of this process on chromatin organization remain largely undefined. Using quantitative middle-down proteomics we demonstrate that H3.3 accumulates to near saturation levels in the chromatin of various mouse somatic tissues by late adulthood. Accumulation of H3.3 is associated with profound changes in global levels of both individual and combinatorial H3 methyl modifications. A subset of these modifications exhibit distinct relat

SUBMITTER: Tvardovskiy A 

PROVIDER: S-EPMC5766163 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature