Model-driven discovery of long-chain fatty acid metabolic reprogramming in heterogeneous prostate cancer cells.

ABSTRACT: Epithelial-mesenchymal-transition promotes intra-tumoral heterogeneity, by enhancing tumor cell invasiveness and promoting drug resistance. We integrated transcriptomic data for two clonal subpopulations from a prostate cancer cell line (PC-3) into a genome-scale metabolic network model to explore their metabolic differences and potential vulnerabilities. In this dual cell model, PC-3/S cells express Epithelial-mesenchymal-transition markers and display high invasiveness and low metastatic potential, while PC-3/M cells present the opposite phenotype and higher proliferative rate. Model-driven analysis and experimental validations unveiled a marked metabolic reprogramming in long-chain fatty acids metabolism. While PC-3/M cells showed an enhanced entry of long-chain fatty acids into the mit

SUBMITTER: Marin de Mas I 

PROVIDER: S-EPMC5766231 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature