Molecular characterization of 5' UTR of the lycopene epsilon cyclase (lcyE) gene among exotic and indigenous inbreds for its utilization in maize biofortification.

ABSTRACT: Maize grains are the important source of food and energy, but possess very low proA (< 2.5 µg/g) compared to target level of 15 µg/g set by HarvestPlus to alleviate VAD. Favorable allele having variation in 5' untranslated region (UTR) of lycopene epsilon cyclase (lcyE) gene enhances concentration of proA in maize. To identify the sequence variation in 5' UTR of lcyE, a set of diverse 13 inbreds of indigenous and exotic origin was characterized for allelic constitution of lcyE. Inbreds possessed wide variation in proA (1.62-23.12 µg/g) with a mean of 9.64 µg/g. The proA in CIMMYT-HarvestPlus genotypes having favorable allele of lcyE was very high (22.28 µg/g), whereas the Indian inbreds with the same allele possessed very low proA (2.48 µg/g). Eight genotypes viz., HKI161, HKI163, HKI161-P

SUBMITTER: Zunjare RU 

PROVIDER: S-EPMC5766451 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature