Beta-catenin cleavage enhances transcriptional activation.

ABSTRACT: Nuclear activation of Wnt/β-catenin signaling is required for cell proliferation in inflammation and cancer. Studies from our group indicate that β-catenin activation in colitis and colorectal cancer (CRC) correlates with increased nuclear levels of β-catenin phosphorylated at serine 552 (pβ-Cat⁵⁵²). Biochemical analysis of nuclear extracts from cancer biopsies revealed the existence of low molecular weight (LMW) pβ-Cat⁵⁵², increased to the exclusion of full size (FS) forms of β-catenin. LMW β-catenin lacks both termini, leaving residues in the armadillo repeat intact. Further experiments showed that TCF4 predominantly binds LMW pβ-Cat⁵⁵² in the nucleus of inflamed and cancerous cells. Nuclear chromatin bound localization of LMW pβ-Cat⁵⁵² was blo

SUBMITTER: Goretsky T 

PROVIDER: S-EPMC5766502 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature