Architecture of a mammalian glomerular domain revealed by novel volume electroporation using nanoengineered microelectrodes.

ABSTRACT: Dense microcircuit reconstruction techniques have begun to provide ultrafine insight into the architecture of small-scale networks. However, identifying the totality of cells belonging to such neuronal modules, the "inputs" and "outputs," remains a major challenge. Here, we present the development of nanoengineered electroporation microelectrodes (NEMs) for comprehensive manipulation of a substantial volume of neuronal tissue. Combining finite element modeling and focused ion beam milling, NEMs permit substantially higher stimulation intensities compared to conventional glass capillaries, allowing for larger volumes configurable to the geometry of the target circuit. We apply NEMs to achieve near-complete labeling of the neuronal network associated with a genetically identified olfactory g

SUBMITTER: Schwarz D 

PROVIDER: S-EPMC5766516 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature