Involvement of Gβγ subunits of G_i protein coupled with S1P receptor on multivesicular endosomes in F-actin formation and cargo sorting into exosomes.

ABSTRACT: Exosomes play a critical role in cell-to-cell communication by delivering cargo molecules to recipient cells. However, the mechanism underlying the generation of the exosomal multivesicular endosome (MVE) is one of the mysteries in the field of endosome research. Although sphingolipid metabolites such as ceramide and sphingosine 1-phosphate (S1P) are known to play important roles in MVE formation and maturation, the detailed molecular mechanisms are still unclear. Here, we show that Rho family GTPases, including Cdc42 and Rac1, are constitutively activated on exosomal MVEs and are regulated by S1P signaling as measured by fluorescence resonance energy transfer (FRET)-based conformational changes. Moreover, we detected S1P signaling-induced filamentous actin (F-actin) formation. A selective

SUBMITTER: Kajimoto T 

PROVIDER: S-EPMC5766922 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature