MicroRNA-874-mediated inhibition of the major G₁/S phase cyclin, CCNE1, is lost in osteosarcomas.

ABSTRACT: The tumor microenvironment is characterized by nutrient-deprived conditions in which the cancer cells have to adapt for survival. Serum starvation resembles the growth factor deprivation characteristic of the poorly vascularized tumor microenvironment and has aided in the discovery of key growth regulatory genes and microRNAs (miRNAs) that have a role in the oncogenic transformation. We report here that miR-874 down-regulates the major G₁/S phase cyclin, cyclin E1 (CCNE1), during serum starvation. Because the adaptation of cancer cells to the tumor microenvironment is vital for subsequent oncogenesis, we tested for miR-874 and CCNE1 interdependence in osteosarcoma cells. We observed that miR-874 inhibits CCNE1 expression in primary osteoblasts, but in aggressive osteosarcomas, m

SUBMITTER: Ghosh T 

PROVIDER: S-EPMC5766939 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature