Determination of the native features of the exoglucanase Cel48S from Clostridium thermocellum.

ABSTRACT:

Background

Clostridium thermocellum is considered one of the most efficient natural cellulose degraders because of its cellulosomal system. As the major exoglucanase of cellulosome in C. thermocellum, Cel48S plays key roles and influences the activity and features of cellulosome to a great extent. Thus, it is of great importance to reveal the enzymatic features of Cel48S. However, Cel48S has not been well performed due to difficulties in purifying either recombinant or native Cel48S proteins.

Results

We observed that the soluble fraction of the catalytic domain of Cel48S (Cel48S_CD) obtained by heterologous expression in Escherichia coli and denaturation-refolding treatment contained a large portion of incorrectly folded proteins with low activity. Using

SUBMITTER: Liu YJ 

PROVIDER: S-EPMC5766998 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature