A-GAME: improving the assembly of pooled functional metagenomics sequence data.

ABSTRACT:

Background

Expression screening of environmental DNA (eDNA) libraries is a popular approach for the identification and characterization of novel microbial enzymes with promising biotechnological properties. In such "functional metagenomics" experiments, inserts, selected on the basis of activity assays, are sequenced with high throughput sequencing technologies. Assembly is followed by gene prediction, annotation and identification of candidate genes that are subsequently evaluated for biotechnological applications.

Results

Here we present A-GAME (A GAlaxy suite for functional MEtagenomics), a web service incorporating state of the art tools and workflows for the analysis of eDNA sequence data. We illustrate the potential of A-GAME workflows using real functional metagenomic

SUBMITTER: Chiara M 

PROVIDER: S-EPMC5767027 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature