Genetic Transformation System for Woody Plant Tripterygium wilfordii and Its Application to Product Natural Celastrol.

ABSTRACT: Tripterygium wilfordii is a perennial woody liana medicinal plant with several crucial biological activities. Although studies on tissue culture have previously been conducted, research on genetic transformation is much more challenging and therefore results in slower progress. In the present study, a highly efficient transformation system involving the particle bombardment of T. wilfordii with the reporter egfp gene using the PDS-1000/He system was established. A total of seven parameters affecting the genetic transformation were investigated using an L₁₈ (6 × 3⁶)-type orthogonal array. The result indicated that DNA delivery conditions of 3-cm target distance, 1100 psi helium pressure, 28 mmHg chamber vacuum pressure, three times number of bombar

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC5767223 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature