Molecular genetic analysis of the melanoma regulatory locus in Xiphophorus interspecies hybrids.

ABSTRACT: Development of spontaneous melanoma in Xiphophorus interspecies backcross hybrid progeny, (X. hellerii × [X. maculatus Jp 163 A × X. hellerii]) is due to Mendelian segregation of a oncogene (xmrk) and a molecularly uncharacterized locus, called R(Diff), on LG5. R(Diff) is thought to suppresses the activity of xmrk in healthy X. maculatus Jp 163 A parental species that rarely develop melanoma. To better understand the molecular genetics of R(Diff), we utilized RNA-Seq to study allele-specific gene expression of spontaneous melanoma tumors and corresponding normal skin samples derived from 15 first generation backcross (BC₁ ) hybrids and 13 fifth generation (BC₅ ) hybrids. Allele-specific expression was determined for all genes and assigned to parental allele inheritanc

SUBMITTER: Lu Y 

PROVIDER: S-EPMC5767473 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature