Hemicastration induced spermatogenesis-related DNA methylation and gene expression changes in mice testis.

ABSTRACT:

Objective

Hemicastration is a unilateral orchiectomy to remove an injured testis, which can induce hormonal changes and compensatory hypertrophy of the remaining testis, and may influence spermatogenesis. However, the underlying molecular mechanisms are poorly understood. Here, we investigated the impact of hemicastration on remaining testicular function.

Methods

Prepubertal mice (age 24 days) were hemicastrated, and their growth was monitored until they reached physical maturity (age 72 days). Subsequently, we determined testis DNA methylation patterns using reduced representation bisulfite sequencing of normal and hemicastrated mice. Moreover, we profiled the testicular gene expression patterns by RNA sequencing (RNA-seq) to examine whether methylation changes affected gen

SUBMITTER: Wang Y 

PROVIDER: S-EPMC5767500 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature