Elevated plasma 8-iso-prostaglandin F_2α levels in human smokers originate primarily from enzymatic instead of non-enzymatic lipid peroxidation.

ABSTRACT: It is widely accepted that free radicals in tobacco smoke lead to oxidative stress and generate the popular lipid peroxidation biomarker 8-iso-prostaglandin F_2α (8-iso-PGF_2α). However, 8-iso-PGF_2α can simultaneously be produced in vivo by the prostaglandin-endoperoxide synthases (PGHS) induced by inflammation. This inflammation-dependent mechanism has never been considered as a source of elevated 8-iso-PGF_2α in tobacco smokers. The goal of this study is to quantify the distribution of chemical- and PGHS-dependent 8-iso-PGF_2α formation in the plasma of tobacco smokers and non-smokers. The influences of gender and hormonal contraceptive use were accounted for. The distribution was determined by measuring the 8-iso-PGF_2α/prosta

SUBMITTER: van 't Erve TJ 

PROVIDER: S-EPMC5767525 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature