A photo-cross-linking approach to monitor folding and assembly of newly synthesized proteins in a living cell.

ABSTRACT: Many proteins form multimeric complexes that play crucial roles in various cellular processes. Studying how proteins are correctly folded and assembled into such complexes in a living cell is important for understanding the physiological roles and the qualitative and quantitative regulation of the complex. However, few methods are suitable for analyzing these rapidly occurring processes. Site-directed in vivo photo-cross-linking is an elegant technique that enables analysis of protein-protein interactions in living cells with high spatial resolution. However, the conventional site-directed in vivo photo-cross-linking method is unsuitable for analyzing dynamic processes. Here, by combining an improved site-directed in vivo photo-cross-linking technique with a pulse-chas

SUBMITTER: Miyazaki R 

PROVIDER: S-EPMC5767871 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature