Screening for Causative Mutations of Major Prolificacy Genes in Iranian Fat-Tailed Sheep.

ABSTRACT:

Background

The presence of different missense mutations in sheep breeds have shown that the bone morphogenetic protein receptor 1B (BMPR1B), bone morphogenetic protein 15 (BMP15) and growth differentiation factor 9 (GDF9) genes play a vital role in ovulation rate and prolificacy in ewes. Therefore, the present study aims to investigate BMPR1B, BMP15 and GDF9 gene mutations in prolific ewes of Iranian fat-tailed Lori-Bakhtiari sheep.

Materials and methods

In the present experimental study, genomic DNA was extracted from whole blood of 10 prolific Lori-Bakhtiari ewes with at least two twinning records in the first four parities to identify point mutations of the BMPR1B, BMP15 and GDF9 genes, using DNA sequencing.

Results

The results obtained from DNA sequencing showed a

SUBMITTER: Abdoli R 

PROVIDER: S-EPMC5767932 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature