Engineering of a DNA Polymerase for Direct m⁶ A Sequencing.

ABSTRACT: Methods for the detection of RNA modifications are of fundamental importance for advancing epitranscriptomics. N⁶ -methyladenosine (m⁶ A) is the most abundant RNA modification in mammalian mRNA and is involved in the regulation of gene expression. Current detection techniques are laborious and rely on antibody-based enrichment of m⁶ A-containing RNA prior to sequencing, since m⁶ A modifications are generally "erased" during reverse transcription (RT). To overcome the drawbacks associated with indirect detection, we aimed to generate novel DNA polymerase variants for direct m⁶ A sequencing. Therefore, we developed a screen to evolve an RT-active KlenTaq DNA polymerase variant that sets a mark for N⁶ -methylation. We identifie

SUBMITTER: Aschenbrenner J 

PROVIDER: S-EPMC5768020 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature