Biocatalytic Oxidative Cascade for the Conversion of Fatty Acids into α-Ketoacids via Internal H₂ O₂ Recycling.

ABSTRACT: The functionalization of bio-based chemicals is essential to allow valorization of natural carbon sources. An atom-efficient biocatalytic oxidative cascade was developed for the conversion of saturated fatty acids to α-ketoacids. Employment of P450 monooxygenase in the peroxygenase mode for regioselective α-hydroxylation of fatty acids combined with enantioselective oxidation by α-hydroxyacid oxidase(s) resulted in internal recycling of the oxidant H₂ O₂ , thus minimizing degradation of ketoacid product and maximizing biocatalyst lifetime. The O₂ -dependent cascade relies on catalytic amounts of H₂ O₂ and releases water as sole by-product. Octanoic acid was converted under mild conditions in aqueous buffer to 2-oxooctanoic acid in a si

SUBMITTER: Gandomkar S 

PROVIDER: S-EPMC5768024 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature