Single-Molecule Imaging of Proteoglycans in the Pericellular Matrix.

ABSTRACT: The pericellular matrix is a robust, hyaluronan-rich polymer brush-like structure that controls access to the cell surface, and plays an important role in cell adhesion, migration, and proliferation. We report the observation of single bottlebrush proteoglycan dynamics in the pericellular matrix of living chondrocytes. Our investigations show that the pericellular matrix undergoes gross extension on the addition of exogenous aggrecan, and that this extension is significantly in excess of that observed in traditional particle exclusion assays. The mean-square displacement of single, bound proteoglycans increases with distance to cell surface, indicating reduced confinement by neighboring hyaluronan-aggrecan complexes. This is consistent with published data from quantitative particle exclusi

SUBMITTER: Scrimgeour J 

PROVIDER: S-EPMC5768515 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature