Monitoring single-cell gene regulation under dynamically controllable conditions with integrated microfluidics and software.

ABSTRACT: Much is still not understood about how gene regulatory interactions control cell fate decisions in single cells, in part due to the difficulty of directly observing gene regulatory processes in vivo. We introduce here a novel integrated setup consisting of a microfluidic chip and accompanying analysis software that enable long-term quantitative tracking of growth and gene expression in single cells. The dual-input Mother Machine (DIMM) chip enables controlled and continuous variation of external conditions, allowing direct observation of gene regulatory responses to changing conditions in single cells. The Mother Machine Analyzer (MoMA) software achieves unprecedented accuracy in segmenting and tracking cells, and streamlines high-throughput curation with a novel leveraged editing procedur

SUBMITTER: Kaiser M 

PROVIDER: S-EPMC5768764 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature