Microbial Lineages in Sarcoidosis. A Metagenomic Analysis Tailored for Low-Microbial Content Samples.

ABSTRACT:

Rationale

The etiology of sarcoidosis is unknown, but microbial agents are suspected as triggers.

Objectives

We sought to identify bacterial, fungal, or viral lineages in specimens from patients with sarcoidosis enriched relative to control subjects using metagenomic DNA sequencing. Because DNA from environmental contamination contributes disproportionately to samples with low authentic microbial content, we developed improved methods for filtering environmental contamination.

Methods

We analyzed specimens from subjects with sarcoidosis (n = 93), control subjects without sarcoidosis (n = 72), and various environmental controls (n = 150). Sarcoidosis specimens consisted of two independent sets of formalin-fixed, paraffin-embedded lymph node biopsies, BAL, Kveim reagent

SUBMITTER: Clarke EL 

PROVIDER: S-EPMC5768903 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature