Epidermal Wnt signalling regulates transcriptome heterogeneity and proliferative fate in neighbouring cells.

ABSTRACT:

Background

Canonical Wnt/beta-catenin signalling regulates self-renewal and lineage selection within the mammalian epidermis. Although the transcriptional response of keratinocytes that receive a Wnt signal is well characterized, little is known about the mechanism by which keratinocytes in proximity to the Wnt-receiving cell are co-opted to undergo a change in cell fate.

Results

To address this, we perform single-cell RNA-sequencing on mouse keratinocytes co-cultured with and without beta-catenin-activated neighbouring cells. We identify five distinct cell states in cultures that had not been exposed to the beta-catenin stimulus and show that the stimulus redistributes wild-type subpopulation proportions. Using temporal single-cell analysis, we reconstruct the cell fate cha

SUBMITTER: Ghahramani A 

PROVIDER: S-EPMC5769491 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature