Dose reduction of bone morphogenetic protein-2 for bone regeneration using a delivery system based on lyophilization with trehalose.

ABSTRACT:

Introduction

To induce sufficient new bone formation, high doses of bone morphogenetic protein-2 (BMP-2) are applied in regenerative medicine that often induce serious side effects. Therefore, improved treatment strategies are required. Here, we investigate whether the delivery of BMP-2 lyophilized in the presence of trehalose reduced the dose of BMP-2 required for bone regeneration.

Materials and methods

A new growth factor delivery system was fabricated using BMP-2-loaded TiO₂ nanotubes by lyophilization with trehalose (TiO₂-Lyo-Tre-BMP-2). We measured BMP-2 release characteristics, bioactivity, and stability, and determined the effects on the osteogenic differentiation of bone marrow stromal cells in vitro. Additionally, we evaluated the ability of t

SUBMITTER: Zhang X 

PROVIDER: S-EPMC5769568 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature