Renal carcinoma/kidney progenitor cell chimera organoid as a novel tumorigenesis gene discovery model.

ABSTRACT: Three-dimensional (3D) organoids provide a new way to model various diseases, including cancer. We made use of recently developed kidney-organ-primordia tissue-engineering technologies to create novel renal organoids for cancer gene discovery. We then tested whether our novel assays can be used to examine kidney cancer development. First, we identified the transcriptomic profiles of quiescent embryonic mouse metanephric mesenchyme (MM) and of MM in which the nephrogenesis program had been induced ex vivo The transcriptome profiles were then compared to the profiles of tumor biopsies from renal cell carcinoma (RCC) patients, and control samples from the same kidneys. Certain signature genes were identified that correlated in the developmentally induced MM and RCC, including component

SUBMITTER: Xu Q 

PROVIDER: S-EPMC5769601 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature