Proteome Profiling of Developing Murine Lens Through Mass Spectrometry.

ABSTRACT:

Purpose

We previously completed a comprehensive profile of the mouse lens transcriptome. Here, we investigate the proteome of the mouse lens through mass spectrometry-based protein sequencing at the same embryonic and postnatal time points.

Methods

We extracted mouse lenses at embryonic day 15 (E15) and 18 (E18) and postnatal day 0 (P0), 3 (P3), 6 (P6), and 9 (P9). The lenses from each time point were preserved in three distinct pools to serve as biological replicates for each developmental stage. The total cellular protein was extracted from the lens, digested with trypsin, and labeled with isobaric tandem mass tags (TMT) for three independent TMT experiments.

Results

A total of 5404 proteins were identified in the mouse ocular lens in at least one TMT set, 4244 in t

SUBMITTER: Khan SY 

PROVIDER: S-EPMC5769801 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature