C3aR and C5aR1 act as key regulators of human and mouse β-cell function.

ABSTRACT:

Aims

Complement components 3 and 5 (C3 and C5) play essential roles in the complement system, generating C3a and C5a peptides that are best known as chemotactic and inflammatory factors. In this study we characterised islet expression of C3 and C5 complement components, and the impact of C3aR and C5aR1 activation on islet function and viability.

Materials and methods

Human and mouse islet mRNAs encoding key elements of the complement system were quantified by qPCR and distribution of C3 and C5 proteins was determined by immunohistochemistry. Activation of C3aR and C5aR1 was determined using DiscoverX beta-arrestin assays. Insulin secretion from human and mouse islets was measured by radioimmunoassay, and intracellular calcium ([Ca²⁺]i), ATP generation and apoptosi

SUBMITTER: Atanes P 

PROVIDER: S-EPMC5769825 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature