XLSearch: a Probabilistic Database Search Algorithm for Identifying Cross-Linked Peptides.

ABSTRACT: Chemical cross-linking combined with mass spectrometric analysis has become an important technique for probing protein three-dimensional structure and protein-protein interactions. A key step in this process is the accurate identification and validation of cross-linked peptides from tandem mass spectra. The identification of cross-linked peptides, however, presents challenges related to the expanded nature of the search space (all pairs of peptides in a sequence database) and the fact that some peptide-spectrum matches (PSMs) contain one correct and one incorrect peptide but often receive scores that are comparable to those in which both peptides are correctly identified. To address these problems and improve detection of cross-linked peptides, we propose a new database search algorithm, X

SUBMITTER: Ji C 

PROVIDER: S-EPMC5770149 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature