Using intracellular markers to identify a novel set of surface markers for live cell purification from a heterogeneous hIPSC culture.

ABSTRACT: Human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) can provide sources for midbrain dopaminergic (mDA) neural progenitors (NPCs) for cell therapy to treat Parkinson's disease (PD) patients. However, the well-known line-to-cell line variability in the differentiation capacity of individual cell lines needs to be improved for the success of this therapy. To address this issue, we sought to identify mDA NPC specific cell surface markers for fluorescence activated cell sorting (FACS). Through RNA isolation after sorting for NPCs based on staining for cell-specific transcription factors followed by microarray, we identified two positive cell surface markers (CORIN and CD166) and one negative cell surface marker (CXCR4) for mDA NPC sorting. These three markers can enric

SUBMITTER: Paik EJ 

PROVIDER: S-EPMC5770419 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature