Development and optimization of a differentiated airway epithelial cell model of the bovine respiratory tract.

ABSTRACT: Cattle are subject to economically-important respiratory tract infections by various bacterial and viral pathogens and there is an urgent need for the development of more realistic in vitro models of the bovine respiratory tract to improve our knowledge of disease pathogenesis. In the present study, we have optimized the culture conditions in serum-free medium that allow bovine bronchial epithelial cells (BBECs) grown at an air-liquid interface to differentiate into a three-dimensional epithelium that is highly representative of the bovine airway. Epidermal growth factor was required to trigger both proliferation and differentiation of BBECs whilst retinoic acid was also essential for mucociliary differentiation. Triiodothyronine was demonstrated not to be important for the differentiation

SUBMITTER: Cozens D 

PROVIDER: S-EPMC5770467 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature