Precision Electrophile Tagging in Caenorhabditis elegans.

ABSTRACT: Adduction of an electrophile to privileged sensor proteins and the resulting phenotypically dominant responses are increasingly appreciated as being essential for metazoan health. Functional similarities between the biological electrophiles and electrophilic pharmacophores commonly found in covalent drugs further fortify the translational relevance of these small-molecule signals. Genetically encodable or small-molecule-based fluorescent reporters and redox proteomics have revolutionized the observation and profiling of cellular redox states and electrophile-sensor proteins, respectively. However, precision mapping between specific redox-modified targets and specific responses has only recently begun to be addressed, and systems tractable to both genetic manipulation and on-target redox si

SUBMITTER: Long MJC 

PROVIDER: S-EPMC5770885 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature