Two Distinct Fluorescence States of the Ligand-Induced Green Fluorescent Protein UnaG.

ABSTRACT: UnaG is a recently discovered ligand-induced fluorescent protein that utilizes bound bilirubin (BR) as its fluorophore. The fluorescence of the UnaG-BR complex (holoUnaG) compares in quantum efficiency to that of enhanced green fluorescent protein, but it is superior in that the fluorophore formation is instantaneous and not dependent on oxygen; hence, much attention has been paid to UnaG as a new fluorescent probe. However, many important molecular properties of fluorescent probes remain unknown, such as the association/dissociation rates of BR, which determine the stability thereof, and the dispersibility of UnaG in aqueous solutions, which influence the functions of labeled proteins. In this study, we found, in the process of investigating the association rate, that the holoUnaG takes t

SUBMITTER: Shitashima Y 

PROVIDER: S-EPMC5770971 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature