A genetically encoded Ca²⁺ indicator based on circularly permutated sea anemone red fluorescent protein eqFP578.

ABSTRACT:

Background

Genetically encoded calcium ion (Ca²⁺) indicators (GECIs) are indispensable tools for measuring Ca²⁺ dynamics and neuronal activities in vitro and in vivo. Red fluorescent protein (RFP)-based GECIs have inherent advantages relative to green fluorescent protein-based GECIs due to the longer wavelength light used for excitation. Longer wavelength light is associated with decreased phototoxicity and deeper penetration through tissue. Red GECI can also enable multicolor visualization with blue- or cyan-excitable fluorophores.

Results

Here we report the development, structure, and validation of a new RFP-based GECI, K-GECO1, based on a circularly permutated RFP derived from the sea anemone Entacmaea quadricolor. We have characterized the performan

SUBMITTER: Shen Y 

PROVIDER: S-EPMC5771076 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature