Using mitoribosomal profiling to investigate human mitochondrial translation.

ABSTRACT: Background: Gene expression in human mitochondria has various idiosyncratic features. One of these was recently revealed as the unprecedented recruitment of a mitochondrially-encoded tRNA as a structural component of the large mitoribosomal subunit. In porcine particles this is mt-tRNA ^Phe whilst in humans it is mt-tRNA ^Val. We have previously shown that when a mutation in mt-tRNA ^Val causes very low steady state levels, there is preferential recruitment of mt-tRNA ^Phe. We have investigated whether this altered mitoribosome affects intra-organellar protein synthesis. Methods: By using mitoribosomal profiling we have revealed aspects of mitoribosome behaviour with its template mt-mRNA under both normal conditions as well as those whe

SUBMITTER: Gao F 

PROVIDER: S-EPMC5771143 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature