Comparison of diagnostic methods to detect Histoplasma capsulatum in serum and blood samples from AIDS patients.

ABSTRACT:

Background

Although early and rapid detection of histoplasmosis is essential to prevent morbidity and mortality, few diagnostic tools are available in resource-limited areas, especially where it is endemic and HIV/AIDS is also epidemic. Thus, we compared conventional and molecular methods to detect Histoplasma capsulatum in sera and blood from HIV/AIDS patients.

Methodology

We collected a total of 40 samples from control volunteers and patients suspected of histoplasmosis, some of whom were also infected with other pathogens. Samples were then analyzed by mycological, serological, and molecular methods, and stratified as histoplasmostic with (group I) or without AIDS (group II), uninfected (group III), and infected with HIV and other pathogens only (group IV). All patients w

SUBMITTER: Dantas KC 

PROVIDER: S-EPMC5771560 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature