Circulating neutrophil transcriptome may reveal intracranial aneurysm signature.

ABSTRACT:

Background

Unruptured intracranial aneurysms (IAs) are typically asymptomatic and undetected except for incidental discovery on imaging. Blood-based diagnostic biomarkers could lead to improvements in IA management. This exploratory study examined circulating neutrophils to determine whether they carry RNA expression signatures of IAs.

Methods

Blood samples were collected from patients receiving cerebral angiography. Eleven samples were collected from patients with IAs and 11 from patients without IAs as controls. Samples from the two groups were paired based on demographics and comorbidities. RNA was extracted from isolated neutrophils and subjected to next-generation RNA sequencing to obtain differential expressions for identification of an IA-associated signature. Bioinfo

SUBMITTER: Tutino VM 

PROVIDER: S-EPMC5771622 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature