Utility of Single-Cell Genomics in Diagnostic Evaluation of Prostate Cancer.

ABSTRACT: A distinction between indolent and aggressive disease is a major challenge in diagnostics of prostate cancer. As genetic heterogeneity and complexity may influence clinical outcome, we have initiated studies on single tumor cell genomics. In this study, we demonstrate that sparse DNA sequencing of single-cell nuclei from prostate core biopsies is a rich source of quantitative parameters for evaluating neoplastic growth and aggressiveness. These include the presence of clonal populations, the phylogenetic structure of those populations, the degree of the complexity of copy-number changes in those populations, and measures of the proportion of cells with clonal copy-number signatures. The parameters all showed good correlation to the measure of prostatic malignancy, the Gleason score, derive

SUBMITTER: Alexander J 

PROVIDER: S-EPMC5771881 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature