Differential RNA Sequencing Implicates Sulfide as the Master Regulator of S⁰ Metabolism in Chlorobaculum tepidum and Other Green Sulfur Bacteria.

ABSTRACT: The green sulfur bacteria (Chlorobiaceae) are anaerobes that use electrons from reduced sulfur compounds (sulfide, S⁰, and thiosulfate) as electron donors for photoautotrophic growth. Chlorobaculum tepidum, the model system for the Chlorobiaceae, both produces and consumes extracellular S⁰ globules depending on the availability of sulfide in the environment. These physiological changes imply significant changes in gene regulation, which has been observed when sulfide is added to Cba. tepidum growing on thiosulfate. However, the underlying mechanisms driving these gene expression changes, i.e., the specific regulators and promoter elements involved, have not yet been defined. Here, differential RNA sequencing (dRNA-seq) was used to globally

SUBMITTER: Hilzinger JM 

PROVIDER: S-EPMC5772222 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature