The Pore-Forming Protein Gasdermin D Regulates Interleukin-1 Secretion from Living Macrophages.

ABSTRACT: The interleukin-1 (IL-1) family cytokines are cytosolic proteins that exhibit inflammatory activity upon release into the extracellular space. These factors are released following various cell death processes, with pyroptosis being a common mechanism. Recently, it was recognized that phagocytes can achieve a state of hyperactivation, which is defined by their ability to secrete IL-1 while retaining viability, yet it is unclear how IL-1 can be secreted from living cells. Herein, we report that the pyroptosis regulator gasdermin D (GSDMD) was necessary for IL-1β secretion from living macrophages that have been exposed to inflammasome activators, such as bacteria and their products or host-derived oxidized lipids. Cell- and liposome-based assays demonstrated that GSDMD pores were required for

SUBMITTER: Evavold CL 

PROVIDER: S-EPMC5773350 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature