Ectopic models for endochondral ossification: comparing pellet and alginate bead culture methods.

ABSTRACT: Key aspects of native endochondral bone development and fracture healing can be mimicked in mesenchymal stem cells (MSCs) through standard in vitro chondrogenic induction. Exploiting this phenomenon has recently emerged as an attractive technique to engineer bone tissue, however, relatively little is known about the best conditions for doing so. The objective of the present study was to compare the bone-forming capacity and angiogenic induction of hypertrophic cell constructs containing human adipose-derived stem cells (hASCs) primed for chondrogenesis in two different culture systems: high-density pellets and alginate bead hydrogels. The hASC constructs were subjected to 4 weeks of identical chondrogenic induction in vitro, encapsulated in an agarose carrier, and then implanted subcutaneo

SUBMITTER: Weiss-Bilka HE 

PROVIDER: S-EPMC5773408 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature