Universal type/subtype-specific antibodies for quantitative analyses of neuraminidase in trivalent influenza vaccines.

ABSTRACT: Both influenza viral hemagglutinin and neuraminidase can induce protective immune responses in humans. Although the viral hemagglutinin antigens have been quantified in influenza vaccines, the amounts of neuraminidase remain undetermined. Using comprehensive bioinformatics analyses of all neuraminidase sequences, we identified highly conserved and subtype-specific peptide epitopes within each of N1, N2 and type B neuraminidase groups. Mono-specific antibodies generated against these peptides bound to their respective subtype/type only while demonstrating remarkable specificity against the viral neuraminidase sequences without any cross-reactivity with allantoic and cellular proteins. Moreover, the subtype/type-specific antibodies were found not to interfere with one another when a mixture

SUBMITTER: Xu K 

PROVIDER: S-EPMC5773574 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature