Speciation of common Gram-negative pathogens using a highly multiplexed high resolution melt curve assay.

ABSTRACT: The identification of the bacterial species responsible for an infection remains an important step for the selection of antimicrobial therapy. Gram-negative bacteria are an important source of hospital and community acquired infections and frequently antimicrobial resistant. Speciation of bacteria is typically carried out by biochemical profiling of organisms isolated from clinical specimens, which is time consuming and delays the initiation of tailored treatment. Whilst molecular methods such as PCR have been used, they often struggle with the challenge of detecting and discriminating a wide range of targets. High resolution melt analysis is an end-point qPCR detection method that provides greater multiplexing capability than probe based methods. Here we report the design of a high resolu

SUBMITTER: Edwards T 

PROVIDER: S-EPMC5773611 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature