Intrinsic Curvature-Mediated Transbilayer Coupling in Asymmetric Lipid Vesicles.

ABSTRACT: We measured the effect of intrinsic lipid curvature, J₀, on structural properties of asymmetric vesicles made of palmitoyl-oleoyl-phosphatidylethanolamine (POPE; J₀<0) and palmitoyl-oleoyl-phosphatidylcholine (POPC; J₀∼0). Electron microscopy and dynamic light scattering were used to determine vesicle size and morphology, and x-ray and neutron scattering, combined with calorimetric experiments and solution NMR, yielded insights into leaflet-specific lipid packing and melting processes. Below the lipid melting temperature we observed strong interleaflet coupling in asymmetric vesicles with POPE inner bilayer leaflets and outer leaflets enriched in POPC. This lipid arrangement manifested itself by lipids melting cooperatively in both leaflets, and a rearrange

SUBMITTER: Eicher B 

PROVIDER: S-EPMC5773765 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature