Primary explant culture and collagen I substrate enhances corneal endothelial cell morphology.

ABSTRACT:

Objectives

Corneal endothelial cell (CEC) isolation and harvest aim to produce engineered grafts to solve donor corneal tissue shortage. To yield high amounts of CEC maintaining morphological and molecular characteristics, several isolation and culture conditions are reported. Here, we combined direct explant culture, with three different coating conditions and a two-step media approach to compare confluence efficiency, morphology, and specific molecular markers expression.

Data description

Confluence was reached after 2 weeks in the three coating conditions (Matrigel, collagen I, and in uncoated plates) using a two-step approach (proliferative medium without pituitary extract, followed by stabilizer basal medium). Na/K-ATPase and GPC4 markers were detected by immunocytochem

SUBMITTER: Zavala J 

PROVIDER: S-EPMC5774048 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature