In-depth proteomic analysis of boar spermatozoa through shotgun and gel-based methods.

ABSTRACT:

Background

Mature spermatozoa contain numerous epididymal and seminal plasma proteins, which full identification through high-throughput technologies may allow for a better understanding of the sperm biology. Therefore, we conducted a global proteomic analysis of boar spermatozoa through shotgun and gel-based methodologies.

Results

The total proteins were extracted from mature spermatozoa and subjecsted to proteome analyses. Functional analyses of gene ontology representations and pathway enrichments were conducted on the shotgun dataset, followed by immunology and gene expression validations. Shotgun and gel-based approaches allowed the detection of 2728 proteins and 2123 spots, respectively. Approximately 38% and 59% of total proteins were respectively fully and partially

SUBMITTER: Feugang JM 

PROVIDER: S-EPMC5774113 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature