In situ characterization of advanced glycation end products (AGEs) in collagen and model extracellular matrix by solid state NMR.

ABSTRACT: Non-enzymatic glycation of extracellular matrix with (U-¹³C₅)-d-ribose-5-phosphate (R5P), enables in situ 2D ssNMR identification of many deleterious protein modifications and crosslinks, including previously unreported oxalamido and hemiaminal (CH₃-CH(OH)NHR) substructures. Changes in charged residue proportions and distribution may be as important as crosslinking in provoking and understanding harmful tissue changes.

SUBMITTER: Li R 

PROVIDER: S-EPMC5774432 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature