Identification and quantification of plasma calciprotein particles with distinct physical properties in patients with chronic kidney disease.

ABSTRACT: Calciprotein particles (CPP) are solid-phase calcium-phosphate bound to serum protein fetuin-A and dispersed as colloids in the blood. Recent clinical studies indicated that serum CPP levels were increased with decline of renal function and associated with inflammation and vascular calcification. However, CPP assays used in these studies measured only a part of CPP over a certain particle size and density. Here we show that such CPP are mostly artifacts generated during processing of serum samples in vitro. The native CPP in fresh plasma are smaller in size and lower in density than those artifactual CPP, composed of fetuin-A carrying amorphous and/or crystalline calcium-phosphate, and increased primarily with serum phosphate levels. We have identified several physicochemical factors that

SUBMITTER: Miura Y 

PROVIDER: S-EPMC5775250 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature