Autophagy promotes metastasis and glycolysis by upregulating MCT1 expression and Wnt/β-catenin signaling pathway activation in hepatocellular carcinoma cells.

ABSTRACT:

Background

Autophagy is a dynamic physiological process that can generate energy and nutrients for cell survival during stress. Autophagy can regulate the migration and invasive ability in cancer cells. However, the connection between autophagy and metabolism is unclear. Monocarboxylate transporter 1 (MCT1) plays an important role in lactic acid transport and H⁺ clearance in cancer cells, and Wnt/β-catenin signaling can increase cancer cell glycolysis. We investigated whether autophagy promotes glycolysis in hepatocellular carcinoma (HCC) cells by activating the Wnt/β-catenin signaling pathway, accompanied by MCT1 upregulation.

Methods

Autophagic activity was evaluated using western blotting, immunoblotting, and transmission electron microscopy. The underlying mec

SUBMITTER: Fan Q 

PROVIDER: S-EPMC5775607 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature