Two complementary reversed-phase separations for comprehensive coverage of the semipolar and nonpolar metabolome.

ABSTRACT: Although it is common in untargeted metabolomics to apply reversed-phase liquid chromatography (RPLC) and hydrophilic interaction liquid chromatography (HILIC) methods that have been systematically optimized for lipids and central carbon metabolites, here we show that these established protocols provide poor coverage of semipolar metabolites because of inadequate retention. Our objective was to develop an RPLC approach that improved detection of these metabolites without sacrificing lipid coverage. We initially evaluated columns recently released by Waters under the CORTECS line by analyzing 47 small-molecule standards that evenly span the nonpolar and semipolar ranges. An RPLC method commonly used in untargeted metabolomics was considered a benchmarking reference. We found that highly non

SUBMITTER: Naser FJ 

PROVIDER: S-EPMC5776055 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature