Single Molecule Force Spectroscopy to Compare Natural versus Artificial Antibody-Antigen Interaction.

ABSTRACT: Biorecognition is central to various biological processes and finds numerous applications in virtually all areas of chemistry, biology, and medicine. Artificial antibodies, produced by imprinting synthetic polymers, are designed to mimic the biological recognition capability of natural antibodies, while exhibiting superior thermal, chemical, and environmental stability compared to their natural counterparts. The binding affinity of the artificial antibodies to their antigens characterizes the biorecognition ability of these synthetic nanoconstructs and their ability to replace natural recognition elements. However, a quantitative study of the binding affinity of an artificial antibody to an antigen, especially at the molecular level, is still lacking. In this study, using atomic force micr

SUBMITTER: Wang C 

PROVIDER: S-EPMC5776662 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature