Dynamics and mechanisms of intracellular calcium waves elicited by tandem bubble-induced jetting flow.

ABSTRACT: One of the earliest events in cellular mechanotransduction is often an increase in intracellular calcium concentration associated with intracellular calcium waves (ICWs) in various physiologic or pathophysiologic processes. Although cavitation-induced calcium responses are believed to be important for modulating downstream bioeffects such as cell injury and mechanotransduction in ultrasound therapy, the fundamental mechanisms of these responses have not been elucidated. In this study, we investigated mechanistically the ICWs elicited in single HeLa cells by the tandem bubble-induced jetting flow in a microfluidic system. We identified two distinct (fast and slow) types of ICWs at varying degrees of flow shear stress-induced membrane deformation, as determined by different bubble standoff d

SUBMITTER: Li F 

PROVIDER: S-EPMC5776977 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature